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KMID : 0620920130450070004
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Experimental & Molecular Medicine
2013 Volume.45 No. 7 p.4 ~ p.0
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A known expressed sequence tag, BM742401, is a potent lincRNA inhibiting cancer metastasis
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Park Seong-Min
Park Sung-Joon
Kim Hee-Jin
Kwon Oh-Hyung
Kang Tae-Wook
Sohn Hyun-Ahm
Kim Seon-Kyu
Noh Seung-Moo
Song Kyu-Sang
Jang Se-Jin
Kim Yong-Sung
Kim Seon-Young
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Abstract
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Long intergenic non-coding RNAs (lincRNAs) have historically been ignored in cancer biology. However, thousands of lincRNAs have been identified in mammals using recently developed genomic tools, including microarray and high-throughput RNA sequencing (RNA-seq). Several of the lincRNAs identified have been well characterized for their functions in carcinogenesis. Here we performed RNA-seq experiments comparing gastric cancer with normal tissues to find differentially expressed transcripts in intergenic regions. By analyzing our own RNA-seq and public microarray data, we identified 31 transcripts, including a known expressed sequence tag, BM742401. BM742401 was downregulated in cancer, and its downregulation was associated with poor survival in gastric cancer patients. Ectopic overexpression of BM742401 inhibited metastasis-related phenotypes and decreased the concentration of extracellular MMP9. These results suggest that BM742401 is a potential lincRNA marker and therapeutic target.
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KEYWORD
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gastric cancer, high-throughput RNA sequencing, long intergenic non-coding RNA, non-coding RNA, RNA-seq
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